Journal: Cell reports

Article Title: Chromatin Landscape Underpinning Human Dendritic Cell Heterogeneity

doi: 10.1016/j.celrep.2020.108180

Figure Lengend Snippet:

Article Snippet: Anti-human CD127/IL-7R (clone A019D5) Sm149 , Fluidigm , Cat# 3149011B; RRID:AB_2661792.

Techniques: Purification, Recombinant, Produced, SYBR Green Assay, Saline, Lysis, Electron Microscopy, Labeling, Staining, Cell Isolation, Enzyme-linked Immunosorbent Assay, Control, Antibody Labeling, Reverse Transcription, DNA Library Preparation, Generated, Microarray, Software

Journal: Heliyon

Article Title: Atrial fibrillation recurrence after catheter ablation is associated with RAD51 and p63 proteins

doi: 10.1016/j.heliyon.2024.e32874

Figure Lengend Snippet: Images of the microarrays from a representative slide of Explorer antibody microarray (ASB600, Full Moon Biosystems, USA) with 656 antibodies per slide in two replicates for each antibody. Enlarged images of individual samples illustrate the differences in the levels of RAD51 in the serum of a patient with compensated AF of the subgroup M1 (A) versus that in the serum of a patient with AF recurrence of the subgroup M2 (B).

Article Snippet: Images of the microarrays from a representative slide of Explorer antibody microarray (ASB600, Full Moon Biosystems, USA) with 656 antibodies per slide in two replicates for each antibody.

Techniques: Microarray

Journal: Heliyon

Article Title: Atrial fibrillation recurrence after catheter ablation is associated with RAD51 and p63 proteins

doi: 10.1016/j.heliyon.2024.e32874

Figure Lengend Snippet: Images of the microarrays from a representative slide of Explorer antibody microarray (ASB600, Full Moon Biosystems, USA) with 656 antibodies per slide in two replicates for each antibody. Enlarged images of individual samples illustrate the differences in the levels of p63 in the serum of a patient with compensated AF of the subgroup M1 (A) versus that in the serum of a patient with AF recurrence of the subgroup M2 (B).

Article Snippet: Images of the microarrays from a representative slide of Explorer antibody microarray (ASB600, Full Moon Biosystems, USA) with 656 antibodies per slide in two replicates for each antibody.

Techniques: Microarray

Journal: Clinical Proteomics

Article Title: Current applications of antibody microarrays

doi: 10.1186/s12014-018-9184-2

Figure Lengend Snippet: Review of planar antibody microarray technologies and their applications in the field of proteomics. Images were adopted from Servier Medical Art by Servier ( http://www.servier.com/Powerpoint-image-bank ) and modified by the authors under the following terms: CREATIVE COMMONS Attribution 3.0 Unported (CC BY 3.0)

Article Snippet: Fullmoon Biosystems Inc , Prostate cancer , Phosphorylation-specific antibody microarray , 95 , 24009409.

Techniques: Microarray, Modification

Journal: Clinical Proteomics

Article Title: Current applications of antibody microarrays

doi: 10.1186/s12014-018-9184-2

Figure Lengend Snippet: Antibody microarray summary

Article Snippet: Fullmoon Biosystems Inc , Prostate cancer , Phosphorylation-specific antibody microarray , 95 , 24009409.

Techniques: Microarray, Phospho-proteomics, Infection, Biomarker Discovery, Virus

Journal: Biomedicines

Article Title: The Enhanced Expression of ZWILCH Predicts Poor Survival of Adrenocortical Carcinoma Patients.

doi: 10.3390/biomedicines11041233

Figure Lengend Snippet: Figure 1. An overall survival area plot showing different estimates of the causal effect of the ZWILCH expression on survival using different Cox proportional hazards regression models (A). The contour plot illustrates the probability of patient survival over time according to ZWILCH gene expression level in ACC patients from the TCGA database (B).

Article Snippet: Slides were immersed in Target Retrieval Solution, Citrate pH 6.1 (#H-3300-250, Vector, Stuttgart, Germany), and heated in a microwave for 5 min. After that, they were cooled down to room temperature (RT) for 20 min. After endogenous peroxidase activity blocking and incubation with 2.5% normal horse serum, the sections were incubated with anti-ZWILCH polyclonal rabbit antibody (#14281-1-AP, Proteintech, Rosemont, IL, USA) with 1:1000 concentration at 4 ◦C overnight.

Techniques: Expressing, Gene Expression

Journal: Biomedicines

Article Title: The Enhanced Expression of ZWILCH Predicts Poor Survival of Adrenocortical Carcinoma Patients.

doi: 10.3390/biomedicines11041233

Figure Lengend Snippet: Figure 5. General transcriptome profile in groups of 10 patients with the highest expression of the ZWILCH gene (up ZWILCH) compared to 10 patients with the lowest expression of the ZWILCH gene (down ZWILCH) (data from GSE10927). Each dot on the graph represents the mean value of the gene expression level of ten patients belonging to a particular group. The cut-off criteria for identifying differentially expressed genes were established as |fold change| = 1.5 and p-value = 0.05, and are represented by orange dotted lines. Genes above the cut-off lines were categorized as up−regulated (green dots) or down−regulated (red dots). The total number of up−and down−regulated genes can be found in the top right and top left corners, respectively. The plots also indicate the symbols for the seven most differentially expressed genes.

Article Snippet: Slides were immersed in Target Retrieval Solution, Citrate pH 6.1 (#H-3300-250, Vector, Stuttgart, Germany), and heated in a microwave for 5 min. After that, they were cooled down to room temperature (RT) for 20 min. After endogenous peroxidase activity blocking and incubation with 2.5% normal horse serum, the sections were incubated with anti-ZWILCH polyclonal rabbit antibody (#14281-1-AP, Proteintech, Rosemont, IL, USA) with 1:1000 concentration at 4 ◦C overnight.

Techniques: Expressing, Gene Expression

Journal: Biomedicines

Article Title: The Enhanced Expression of ZWILCH Predicts Poor Survival of Adrenocortical Carcinoma Patients.

doi: 10.3390/biomedicines11041233

Figure Lengend Snippet: Figure 8. Gene set enrichment analysis (GSEA) from the comparison of patients with low and high ZWILCH expression, based on GSE10927. Clustering of enriched gene sets into common functional groups, each cluster is marked with a different color (A). The barplot with ten of the most activated and inhibited gene terms according to the normalized enrichment score values (B). Detailed enrichment plots for the five most inhibited and the five most activated gene sets, showing the profile of the running NES score and the positions of the genes on the rank−ordered list (C,D).

Article Snippet: Slides were immersed in Target Retrieval Solution, Citrate pH 6.1 (#H-3300-250, Vector, Stuttgart, Germany), and heated in a microwave for 5 min. After that, they were cooled down to room temperature (RT) for 20 min. After endogenous peroxidase activity blocking and incubation with 2.5% normal horse serum, the sections were incubated with anti-ZWILCH polyclonal rabbit antibody (#14281-1-AP, Proteintech, Rosemont, IL, USA) with 1:1000 concentration at 4 ◦C overnight.

Techniques: Comparison, Expressing, Functional Assay

Journal: Biomedicines

Article Title: The Enhanced Expression of ZWILCH Predicts Poor Survival of Adrenocortical Carcinoma Patients.

doi: 10.3390/biomedicines11041233

Figure Lengend Snippet: Figure 10. The expression of ZWILCH protein in human adrenal gland disease spectrum (adrenal cancer progression) tissue microarray (TMA) slide. The general profile of immunohistochemical staining presents localization of ZWILCH protein (A). TMA map shows the relevant types of adrenal cancer progression marked in an appropriate color (B). Densitometric analysis of ZWILCH gene expression in the tissue array side of the adrenal gland disease spectrum. The boxplot displays each group with its median and interquartile range (IQR) (C). Individual patient densitometric data were added to the corresponding boxplots and represented as dots. The Kruskal–Wallis (KW) test was used to compare groups, followed by the Dunn post hoc test. Differences between groups were marked with asterisks: ** for p < 0.01. Letter annotation was used to indicate statistically significant differences (p < 0.05) between compared groups. Paired comparisons with post hoc Dunn p-values are also shown in the table.

Article Snippet: Slides were immersed in Target Retrieval Solution, Citrate pH 6.1 (#H-3300-250, Vector, Stuttgart, Germany), and heated in a microwave for 5 min. After that, they were cooled down to room temperature (RT) for 20 min. After endogenous peroxidase activity blocking and incubation with 2.5% normal horse serum, the sections were incubated with anti-ZWILCH polyclonal rabbit antibody (#14281-1-AP, Proteintech, Rosemont, IL, USA) with 1:1000 concentration at 4 ◦C overnight.

Techniques: Expressing, Microarray, Immunohistochemical staining, Staining, Gene Expression

Journal: Biomedicines

Article Title: The Enhanced Expression of ZWILCH Predicts Poor Survival of Adrenocortical Carcinoma Patients.

doi: 10.3390/biomedicines11041233

Figure Lengend Snippet: Figure 11. Representative immunostaining of adrenal gland disease spectrum (adrenal cancer pro- gression) TMA slide. Brown staining (B,C,E,F,H,I,K,L,N,O) indicates ZWILCH protein (red arrows), located typically cellular with hematoxylin counterstain (nucleus). The negative control of adrenal gland tissue (A,D,G,J,L,M). Original magnification was 100× (A,B,D,E,G,H,M,N), 200× (J,L,K), and 400× (C,F,I,L,O).

Article Snippet: Slides were immersed in Target Retrieval Solution, Citrate pH 6.1 (#H-3300-250, Vector, Stuttgart, Germany), and heated in a microwave for 5 min. After that, they were cooled down to room temperature (RT) for 20 min. After endogenous peroxidase activity blocking and incubation with 2.5% normal horse serum, the sections were incubated with anti-ZWILCH polyclonal rabbit antibody (#14281-1-AP, Proteintech, Rosemont, IL, USA) with 1:1000 concentration at 4 ◦C overnight.

Techniques: Immunostaining, Staining, Negative Control

Journal: Therapeutic Advances in Medical Oncology

Article Title: SOX11: friend or foe in tumor prevention and carcinogenesis?

doi: 10.1177/1758835919853449

Figure Lengend Snippet: The molecular structure of SOX11. The SOX11 protein is composed of 441 amino acids. SOX11 contains two functional domains, the N-terminal HMG domain and the conserved TAD. SOX11, sex-determining region Y-related high-mobility-group box transcription factor 11; HMG, high mobility group; TAD, C-terminal transactivation domain.

Article Snippet: MCL , 53 , IHC , Rabbit polyclonal (Atlas Antibodies AB) , SOX11-negative patients show worse prognosis and shorter OS. , Wang et al. .

Techniques: Functional Assay

Journal: Therapeutic Advances in Medical Oncology

Article Title: SOX11: friend or foe in tumor prevention and carcinogenesis?

doi: 10.1177/1758835919853449

Figure Lengend Snippet: Overview of SOX11 function reported in diverse types of cancer.

Article Snippet: MCL , 53 , IHC , Rabbit polyclonal (Atlas Antibodies AB) , SOX11-negative patients show worse prognosis and shorter OS. , Wang et al. .

Techniques: Expressing, Over Expression, Biomarker Discovery, Migration, Methylation, DNA Methylation Assay

Journal: Therapeutic Advances in Medical Oncology

Article Title: SOX11: friend or foe in tumor prevention and carcinogenesis?

doi: 10.1177/1758835919853449

Figure Lengend Snippet: Overall carcinogenic actions of SOX11 on the hallmarks of tumor biology. SOX11 exerts tumor-stimulative effects through increasing cell proliferation, repressing cell differentiation, inducing angiogenesis, and promoting metastasis. BCL6, B-cell lymphoma 6; BNIP3, B-cell lymphoma 2 (BCL2)/adenovirus E1B 19 kDa protein-interacting protein 3; CIC, cancer-initiating cell; PAX5, Paired box protein 5; PDGFA, platelet-derived growth factor A; SETMAR, SET domain and mariner transposase fusion gene; SOX11, Sex-determining region Y-related high-mobility-group box transcription factor 11; TANK, TRAF family member-associated NF-κB activator.

Article Snippet: MCL , 53 , IHC , Rabbit polyclonal (Atlas Antibodies AB) , SOX11-negative patients show worse prognosis and shorter OS. , Wang et al. .

Techniques: Cell Differentiation, Derivative Assay

Journal: Therapeutic Advances in Medical Oncology

Article Title: SOX11: friend or foe in tumor prevention and carcinogenesis?

doi: 10.1177/1758835919853449

Figure Lengend Snippet: Different prognostic significance of SOX11 in tumor cases.

Article Snippet: MCL , 53 , IHC , Rabbit polyclonal (Atlas Antibodies AB) , SOX11-negative patients show worse prognosis and shorter OS. , Wang et al. .

Techniques: Expressing, Microarray, Over Expression

Journal: NPJ Vaccines

Article Title: Epitope-specific immunity against Staphylococcus aureus coproporphyrinogen III oxidase

doi: 10.1038/s41541-020-00268-2

Figure Lengend Snippet: a S. aureus CgoX structure was modulated from B. subtillis (3I6D.pdb). The linear epitope of mAb D3 (red) was identified by microarray technology using overlapping 13mer CgoX peptides (Supplementary Fig. ). Protein structure was visualised by EzMol2.1. b Alanine scan of epitope peptides for binding analysis of anti-CgoX mAb D3. Single amino acid positions of the D3 epitope were consecutively replaced by alanine (red in right panel). Immobilised peptides were stained by anti-CgoX mAb D3 detected with anti-mIgG-HRP. Data are presented as mean ± s.d. ( n = 2 technical replicates). c Allele frequencies of anti-CgoX mAb D3 epitope. Genome sequences of S. aureus clinical isolates were analysed for epitope aa sequence using the RidomSeqsphere core genome multi locus sequence typing (cgMLST) database. Amino acids interacting with paratope of anti-CgoX mAb D3 according to alanine scan are marked in red. Amino acid differences from identified epitope peptide sequence are marked in blue. Frequencies of alleles with non-restricted binding of anti-CgoX mAb D3 are marked in green. d Uniqueness of the CgoX D3 epitope in S. aureus . Sequence alignment of CgoX from S. aureus with PPOX from H. sapiens and M. musculus . CgoX D3 epitope is depicted in yellow. e Competition analysis of CgoX mAb. Binding of DyLight-649-conjugated anti-CgoX mAb D3 to rCgoX was competed for with different concentrations of unconjugated, indicated mAbs and analysed by ELISA. Binding was determined by fluorescence measurement (Ex 646/Em 674). Data are presented as mean ± s.d. ( n = 2). f Saturation binding curve was generated by plotting absorbance signals (OD 450nm ) of increasing amounts of anti-CgoX huMAb D3 to rCgoX coated on ELISA MaxiSorp plate using the GraphPadPrism 8.4 software. Kd was calculated by non-linear fitting and the equation for one-site binding model [Y = Bmax*X/(Kd + X)].

Article Snippet: Upon washing with TBST (3 × 15 min), HRP conjugated secondary goat anti-mouse IgG (Sigma-Aldrich, Germany, cat. No. A3673) or goat anti-human IgG antibody (Bio-Rad, Germany, cat. No. 172-1050) were diluted 1:3,000 and applied for 1 h at room temperature, respectively.

Techniques: Microarray, Binding Assay, Staining, Sequencing, Enzyme-linked Immunosorbent Assay, Fluorescence, Generated, Software

Journal: NPJ Vaccines

Article Title: Epitope-specific immunity against Staphylococcus aureus coproporphyrinogen III oxidase

doi: 10.1038/s41541-020-00268-2

Figure Lengend Snippet: a Competition ELISA. Binding of anti-CgoX mAb D3 to rCgoX coated on ELISA MaxiSorp plate was competed for with CgoX-D3-BSA conjugate. Binding was detected with anti-mIgG-HRP and compared to control sample (BSA). b Anti-CgoX-BSA IgG titer. Sera of two CgoX-D3-BSA immunised mice were collected at day 68 and analysed together with preimmune serum for anti-CgoX IgGs by ELISA. c Survival of mice challenged with S. aureus upon immunisation with CgoX-D3 epitope peptide conjugated with BSA. BALB/c mice ( n = 11) immunised with CgoX-D3-BSA or the carrier protein BSA (black) as control group ( n = 10), were infected i.p. with 3.3 × 10 7 cfu S. aureus USA300 mixed with 5% mucin from porcine stomach. Significance was calculated by Log-rank (Mantel-Cox) test.

Article Snippet: Upon washing with TBST (3 × 15 min), HRP conjugated secondary goat anti-mouse IgG (Sigma-Aldrich, Germany, cat. No. A3673) or goat anti-human IgG antibody (Bio-Rad, Germany, cat. No. 172-1050) were diluted 1:3,000 and applied for 1 h at room temperature, respectively.

Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay, Control, Infection